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Soy and legumes from Eastern Himalayas — Sikkim and Darjeeling in India, eastern Nepal and Bhutan

Kinemaकिनेमा

Kinema is the sticky fermented soybean of the eastern Himalayas. Soybeans are soaked overnight, boiled soft and drained, then wrapped in leaves in a bamboo basket above the kitchen and left to ferment naturally for one to three days. Bacillus bacteria coat the beans in a whitish, sticky film with a mild ammoniacal flavour, and kinema is eaten as a curry with rice.

Fully documentedSoy and legumes26 h – 3.1 days

Recipe

Kinema, a photo from Wikimedia Commons
Photo: Kaajakocks, CC BY-SA 3.0, via Wikimedia Commons
Amounts from a sourceHousehold craft with no fixed amounts. The times, temperature and the 1 % firewood ash below come from one laboratory preparation (Khadka et al. 2024).
SoybeansLocal varieties[Kharnaior2022]
Firewood ash1 % firewood ashMixed into the cracked beans in a laboratory preparation; the other sources do not give an amount[Khadka2024]
Starterspontaneous; the microbes come from the soybeans, the equipment, the firewood ash and the wrapping materials

Total time across the recorded steps: 26 h – 3.1 days

Start
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Close-up: water moving into a bean

What changes: Soybeans are washed and soak overnight

Step 1 of 6
Select, wash and soak the soybeans
Time
Overnight
Reported
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Bacillus

Output: soaked beans.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Kinema is the sticky fermented soybean of the eastern Himalayas. Soybeans are soaked overnight, boiled soft and drained, then wrapped in leaves in a bamboo basket above the kitchen and left to ferment naturally for one to three days. Bacillus bacteria coat the beans in a whitish, sticky film with a mild ammoniacal flavour, and kinema is eaten as a curry with rice. Made in 6 steps over 26 h – 3.1 days. The longest step, wrap and ferment in a leaf-lined basket above the kitchen, takes 24 h – 3 days (Kharnaior P 2022) and runs at 28–32 °C (Khadka D 2024). Bacillus subtilis and Bacillus licheniformis and B. sonorensis do most of the fermenting.

Key numbers
Main step24 h – 3 daysReported[Kharnaior2022][Khadka2024]Wrap and ferment in a leaf-lined basket above the kitchen
Temperature28–32 °CReported[Khadka2024]Wrap and ferment in a leaf-lined basket above the kitchen

What makes this one different

Kinema is fermented naturally, without a starter. In one laboratory preparation the cracked beans were mixed with firewood ash and wrapped in leaves. Its stickiness is presumably poly-γ-glutamic acid made by Bacillus [Khadka2024, Chettri2016, Kharnaior2022].

Related ferments

Safety

Bacillus cereus is a food-borne pathogen whose effects range from harmless to lethal, and it was the abundant Bacillus in kinema from Bhutan [Kharnaior2022]. A metagenomic study of Sikkim kinema also detected B. cereus, Staphylococcus aureus and Proteus mirabilis in some seasons [Kumar2019]. The sources set no numerical limit.

Hazards the sources name: Bacillus cereus; Staphylococcus aureus and Proteus mirabilis (detected in some seasons by metagenomic sequencing).

History and sources

Every number is labelled with how it is known. How this atlas is made

Kharnaior and Tamang (2022) say kinema has been eaten in the eastern Himalayan regions of India, Nepal and Bhutan for more than 2,000 years, citing Tamang and Samuel (2010).

  1. [Kharnaior2022] Kharnaior P., Tamang J.P. 2022, Metagenomic-metabolomic mining of kinema, a naturally fermented soybean food of the Eastern Himalayas, Frontiers in Microbiology 13:868383, doi:10.3389/fmicb.2022.868383paper
  2. [Khadka2024] Khadka D., Pahadi T., Aryal S., Karki D. 2024, Partial purification and characterization of protease extracted from kinema, Heliyon 10:e27173, doi:10.1016/j.heliyon.2024.e27173paper
  3. [Chettri2016] Chettri R., Bhutia M.O., Tamang J.P. 2016, Poly-γ-glutamic acid (PGA)-producing Bacillus species isolated from kinema, Indian fermented soybean food, Frontiers in Microbiology 7:971, doi:10.3389/fmicb.2016.00971paper
  4. [Kumar2019] Kumar J., Sharma N., Kaushal G., Samurailatpam S., Sahoo D., Rai A.K., Singh S.P. 2019, Metagenomic insights into the taxonomic and functional features of kinema, a traditional fermented soybean product of Sikkim Himalaya, Frontiers in Microbiology 10:1744, doi:10.3389/fmicb.2019.01744paper
  5. [Thapa2021] Thapa P., Thapa A., Khadka S., Sapkota S., Panta O.P., Sharma S., Karki T.B., Poudel P. 2021, Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from kinema, water and soil samples, Heliyon 7:e07715, doi:10.1016/j.heliyon.2021.e07715paper