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Soy and legumes from Huai River basin

Chouyandou, 'stinky salt beans' (Huai River natto-like soybean)臭盐豆

Chouyandou is a sticky, strong-smelling Bacillus soybean from the Huai River basin, close to Japanese natto. Soaked yellow soybeans are steamed, seeded with Bacillus subtilis or simply wrapped hot in cloth and buried in straw, and kept warm until they grow a white film and long threads. The beans are seasoned to taste, after-ripened in the cold and eaten fresh or dried.

DocumentedSoy and legumes27 h – 2.9 days

Recipe

Amounts from a sourceThe farmhouse method in a 2023 draft group standard; factories spray about 1 ml of culture per kg of raw beans instead.
Yellow soybeans500 gSorted[CGAPA-cyd]
Bacillus subtilis culture5 gOptional; dissolved in 50 ml of water at 38–45 °C[CGAPA-cyd]
Salt, chilli and other seasoningsAdded to taste when the beans are taken out[CGAPA-cyd]
Starterdefined B. subtilis culture, or none in the straw-nest (wudou) method

Total time across the recorded steps: 27 h – 2.9 days

Start
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Close-up: water moving into a bean

What changes: Soybeans soak until they double in weight

Step 1 of 6
Soak
Time
7–24 h
Reported
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
B. subtilis
Bacillus subtilis

Output: soaked beans.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Chouyandou is a sticky, strong-smelling Bacillus soybean from the Huai River basin, close to Japanese natto. Soaked yellow soybeans are steamed, seeded with Bacillus subtilis or simply wrapped hot in cloth and buried in straw, and kept warm until they grow a white film and long threads. The beans are seasoned to taste, after-ripened in the cold and eaten fresh or dried. Made in 6 steps over 27 h – 2.9 days. The longest step, keep warm until stringy, takes 14–36 h (T/CGAPA group standard 2023) and runs at 35–52 °C (T/CGAPA group standard 2023). Bacillus subtilis does most of the fermenting.

Key numbers
Main step14–36 hReported[CGAPA-cyd]Keep warm until stringy
Temperature35–52 °CReported[CGAPA-cyd]Keep warm until stringy

What makes this one different

Chouyandou is made like natto, but farm households traditionally made it only in the cold season and could ferment it with no starter by burying the hot beans in straw.

Related ferments

Variants

  • Wudou (straw-nest method, no starter)Hot beans wrapped in white cotton cloth or a new clay basin and buried in kitchen straw, wheat-ear straw being best, at 20–30 °C for 3–4 h as written in the draft [CGAPA-cyd].

Safety

A 2023 consultation draft of a group standard says factory wet chouyandou must stay at 5 °C or below through storage and sale, with a 3-month shelf life, and must meet China's soybean-product standard GB 2712 [CGAPA-cyd]. In a DNA sequencing study of pooled samples, B. cereus was abundant in kinema from Bhutan, a related Himalayan Bacillus soybean food, while B. subtilis led in kinema from India and Nepal; the study did not test chouyandou (Kharnaior and Tamang 2022).

Hazards the sources name: Bacillus cereus, abundant by DNA sequencing of pooled kinema from Bhutan, a related Himalayan Bacillus soybean ferment (not studied in chouyandou).

History and sources

Every number is labelled with how it is known. How this atlas is made

Farm households make it only from Shuangjiang (frost's descent, late October) to Lichun (start of spring, early February) [CGAPA-cyd].

  1. [CGAPA-cyd] T/CGAPA group standard, consultation draft (2023), 臭盐豆制作技术规程 (technical specification for production of stinky salt bean), China Association for Development of Quality Agricultural Productsspec
  2. [Kharnaior2022] Kharnaior P., Tamang J.P. 2022, Metagenomic-metabolomic mining of kinema, a naturally fermented soybean food of the Eastern Himalayas, Frontiers in Microbiology 13:868383paper