Skip to main content
Support

Browse by category

All categories
Home

Insects from China

Fermented silkworm pupae seasoning (modern)

This is a modern lab seasoning made from silkworm (Bombyx mori) pupae. A 2025 Chinese study fermented the pupae with Latilactobacillus sakei at 32 °C for 10 days, which raised the monosodium glutamate (MSG) equivalent 6.74-fold. Earlier Japanese studies made liquid seasonings from pupae with protease (2009) or koji (2011), with or without chitinase, aged 90 days.

Fully documentedInsects10 days

Recipe

Amounts from a sourceOptimised lab conditions from a 2025 response-surface study (abstract); the abstract does not give batch size, water or salt.
Silkworm pupae[qin2025]
Latilactobacillus sakei (Lactobacillus sakei) culture1.0 % inoculumThe optimum among the bacterial species the study compared[qin2025]
StarterLatilactobacillus sakei (Lactobacillus sakei) culture added at 1.0 % inoculum

Total time across the recorded steps: 10 days

Start
Drag the bar or press Play

What changes: Starting material: silkworm pupae

Step 1 of 3
Start with silkworm pupae
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Latilactobacillus sakei
Moulds
Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

This is a modern lab seasoning made from silkworm (Bombyx mori) pupae. A 2025 Chinese study fermented the pupae with Latilactobacillus sakei at 32 °C for 10 days, which raised the monosodium glutamate (MSG) equivalent 6.74-fold. Earlier Japanese studies made liquid seasonings from pupae with protease (2009) or koji (2011), with or without chitinase, aged 90 days. Made in 3 steps over 10 days. The longest step, ferment warm, takes 10 days (Qin F 2025) and runs at 32 °C (Qin F 2025). Latilactobacillus sakei and Koji mould do most of the fermenting.

Key numbers
Main step10 daysReported[qin2025]Ferment warm
Temperature32 °CReported[qin2025]Ferment warm

What makes this one different

Unlike a soy or fish sauce, this seasoning uses insect pupae as the protein and, in the 2025 study, a lactic starter instead of koji. The 2011 Japanese version used koji and was aged 90 days; the 2009 version used protease, not koji.

Related ferments

Variants

  • Koji and chitinase seasoning (Japan, 2011)Pupae made into a seasoning with koji, with or without chitinase, aged 90 days; total nitrogen 1.69–1.70 g/100 g, about twice the free amino acids of soy sauce and about 23 mg/100 ml GABA. It was clear and light brown.
  • Protease and chitinase seasoning (Japan, 2009)Made with protease, with or without chitinase, aged 90 days; total nitrogen 1.07–1.08 g/100 g, clear and light yellow.

Safety

This is a lab product, not a traditional food. Silkworm pupa protein is highly allergenic; one study found lactic fermentation cut its IgE binding by 22 %, which lowers but does not remove the risk, so treat the seasoning as an allergen.

History and sources

Every number is labelled with how it is known. How this atlas is made

A 2009 Japanese study made a liquid seasoning from silkworm pupae with protease, with or without chitinase, aged 90 days. A 2011 follow-up made one with koji. In 2025 a Chinese team fermented pupae with Latilactobacillus sakei and optimised the conditions by response-surface methodology.

  1. [qin2025] Qin F., Wang S., Zou Y., Li E., Mu L., Li Q. 2025, Optimization of silkworm pupae fermentation by response surface methodology and products taste characteristics study, Science and Technology of Food Industry (Shipin gongye ke-ji) 46(1):231-238, doi:10.13386/j.issn1002-0306.2024020086 (abstract via DOAJ)paper
  2. [taniguchi2011] Taniguchi Yamada A., Sakamaki M., Kikuchi S., Takano K. 2011, Silkworm pupae in the production of a new liquid seasoning II (さなぎを用いた新規調味液の製造Ⅱ), Food Preservation Science 37(6):295-300, doi:10.5891/jafps.37.295 (abstract)paper
  3. [taniguchi2009] Taniguchi Yamada A., Sakamaki M., Kikuchi S., Takano K. 2009, Silkworm pupae for production of new liquid seasoning I, Food Preservation Science 35(6):285-289, doi:10.5891/jafps.35.285 (abstract)paper
  4. [yang2026] Yang J., Zhou S., Chen Y., Wangtueai S., Song J. 2026, Enhanced functionality and reduced allergenicity of silkworm pupae protein through Lactiplantibacillus plantarum fermentation, Food Chemistry 510:148747 (abstract)paper