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This is a modern lab seasoning made from silkworm (Bombyx mori) pupae. A 2025 Chinese study fermented the pupae with Latilactobacillus sakei at 32 °C for 10 days, which raised the monosodium glutamate (MSG) equivalent 6.74-fold. Earlier Japanese studies made liquid seasonings from pupae with protease (2009) or koji (2011), with or without chitinase, aged 90 days.
Fully documentedInsects10 days
Recipe
Amounts from a sourceOptimised lab conditions from a 2025 response-surface study (abstract); the abstract does not give batch size, water or salt.
The optimum among the bacterial species the study compared[qin2025]
Starter
Latilactobacillus sakei (Lactobacillus sakei) culture added at 1.0 % inoculum
Total time across the recorded steps: 10 days
Start
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What changes:Starting material: silkworm pupae
Step 1 of 3
Start with silkworm pupae
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Latilactobacillus sakei
Moulds
Flavour
Not documented
Texture
Not documented
Colour
Not documented
Microbes at work in this step
Not documented for this step.
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
At a glance
This is a modern lab seasoning made from silkworm (Bombyx mori) pupae. A 2025 Chinese study fermented the pupae with Latilactobacillus sakei at 32 °C for 10 days, which raised the monosodium glutamate (MSG) equivalent 6.74-fold. Earlier Japanese studies made liquid seasonings from pupae with protease (2009) or koji (2011), with or without chitinase, aged 90 days. Made in 3 steps over 10 days. The longest step, ferment warm, takes 10 days (Qin F 2025) and runs at 32 °C (Qin F 2025). Latilactobacillus sakei and Koji mould do most of the fermenting.
Unlike a soy or fish sauce, this seasoning uses insect pupae as the protein and, in the 2025 study, a lactic starter instead of koji. The 2011 Japanese version used koji and was aged 90 days; the 2009 version used protease, not koji.
Koji and chitinase seasoning (Japan, 2011)Pupae made into a seasoning with koji, with or without chitinase, aged 90 days; total nitrogen 1.69–1.70 g/100 g, about twice the free amino acids of soy sauce and about 23 mg/100 ml GABA. It was clear and light brown.
Protease and chitinase seasoning (Japan, 2009)Made with protease, with or without chitinase, aged 90 days; total nitrogen 1.07–1.08 g/100 g, clear and light yellow.
Safety
This is a lab product, not a traditional food. Silkworm pupa protein is highly allergenic; one study found lactic fermentation cut its IgE binding by 22 %, which lowers but does not remove the risk, so treat the seasoning as an allergen.
A 2009 Japanese study made a liquid seasoning from silkworm pupae with protease, with or without chitinase, aged 90 days. A 2011 follow-up made one with koji. In 2025 a Chinese team fermented pupae with Latilactobacillus sakei and optimised the conditions by response-surface methodology.