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Fermented milk from Bulgaria

Ant yoghurt

Ant yoghurt is a nearly forgotten Balkan and Anatolian way to start yoghurt without a culture. Live red wood ants are dropped into warm milk, and the jar is buried in the ant mound overnight. The ants bring formic acid and lactic and acetic acid bacteria, and by the next day the milk has begun to sour and set.

Fully documentedFermented milk26 h

Recipe

Amounts from a sourceAmounts from a 2023 reconstruction in the village of Nova Mahala, Bulgaria, made with villagers who remembered the practice.
Raw cow's milk400 mLWarmed until nearly scalding, so that it could "bite your pinkie finger"[sinotte2025]
Live red wood ants (Formica rufa)4Added whole; the ants were not crushed in the traditional practice[sinotte2025]
Starterother: live red wood ants (Formica rufa group)

Total time across the recorded steps: 26 h

Start
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What changes: Raw milk is warmed until it is almost too hot to touch

Step 1 of 4
Warm the milk
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Acetic acid bacteria
F. sanfranciscensis

Villagers judged the heat by touch, hot enough to "bite your pinkie finger". Output: milk warmed until nearly scalding.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Ant yoghurt is a nearly forgotten Balkan and Anatolian way to start yoghurt without a culture. Live red wood ants are dropped into warm milk, and the jar is buried in the ant mound overnight. The ants bring formic acid and lactic and acetic acid bacteria, and by the next day the milk has begun to sour and set. Made in 4 steps over 26 h. The longest step, bury the jar in the ant mound overnight, takes 26 h (Sinotte V.M. et al. 2025). Lactic and acetic acid bacteria carried by the ants, including Fructilactobacillus sanfranciscensis and Oecophyllibacter saccharovorans do most of the fermenting.

Key numbers
Main step26 hReported[sinotte2025]Bury the jar in the ant mound overnight
AciditypH 5Measured[sinotte2025]Take the jar out, taste and check

What makes this one different

Ant yoghurt is started with live wood ants instead of old yoghurt. Ant formic acid and proteases help it coagulate at a higher pH than ordinary yoghurt, and the bacteria include F. sanfranciscensis, a sourdough species.

Related ferments

Variants

  • Laboratory ant yoghurt30 mL of sterile milk with 0.5 g of crushed live ants, incubated at 42 °C for 8 h and kept at 4 °C overnight (Sinotte et al. 2025).
  • Ant-egg starter (Anatolian nomads)A 1938 record says nomads short of starter crushed tiny ant eggs in their palms and put them into the milk (Mutlu Sirakova 2023, citing Yalman).

Safety

The study's authors caution against making ant yoghurt unless it is part of your own heritage or you have extensive food-microbiology experience, because it carries several food safety risks. Formica ants can carry the parasite Dicrocoelium dendriticum. In modern kitchens the ants are frozen or put in alcohol to kill it, or the ant starter is strained through a fine sieve, but frozen ants favoured Bacillus. A Danish study on red wood ants found that freezing at -20 or -80 °C for 30 minutes, boiling for 1 minute or storage in 50 % ethanol for 24 hours killed the parasite's cysts (Jensen 2017, abstract). Formica ants are a Novel Food in the European Union and are not approved for sale.

Hazards the sources name: Bacillus spp. (potential food-borne pathogens) in yoghurts started with frozen ants; Dicrocoelium dendriticum, a parasite Formica ants can carry at low prevalence.

History and sources

Every number is labelled with how it is known. How this atlas is made

Three villagers in Bulgaria described burying a can of milk in an ant colony overnight, one from direct experience more than 50 years earlier (Mutlu Sirakova 2023). In 2023 researchers made it again with villagers in Nova Mahala, Bulgaria.

  1. [sinotte2025] Sinotte V.M. et al. 2025, Making yogurt with the ant holobiont uncovers bacteria, acids, and enzymes for food fermentation, iScience 28(10):113595 (read via Europe PMC full text, PMC12570347)paper
  2. [jensen2017] Jensen A.B., Malagocka J., Eilenberg J., Fredensborg B.L. 2017, Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments, Journal of Insects as Food and Feed 3(1):15-20, doi:10.3920/JIFF2016.0042 (abstract, read via Crossref)paper
  3. [mutlu2023] Mutlu Sirakova S. 2023, Forgotten stories of yogurt, cultivating multispecies wisdom, Journal of Ethnobiology (author copy, University of Pécs)paper